1. Knowing the potential of white frangipani flower (Plumeria alba) as a detection of pregnancy in humans.
2. Knowing how to make a test solution of white frangipani flower (Plumeria alba) as a detection of pregnancy in humans?
3. Knowing how of white frangipani flower (Plumeria alba) solution works in detecting pregnancy in humans?
This research uses the following tools:
1. Glass of 100 ml
2. Pipette drops
3. The reaction tube
4. Bunsen burner
6. Legs three
7. Filter paper
8. Plate drops
9. Esbes webs
1. White frangipani flower (Plumeria alba)
2. Ethanol solution 70%
3. Pregnant women urine
4. Women is not pregnant urine
The experimental process was carried out covering the procedure of making white frangium liquid extract (Plumeria alba) and application procedure to urine sample. The details of the manufacturing steps as follows.
1. Pour the urine of pregnant women and non-pregnant mothers into each of the beaker and labeled.
2. Take the urine with a pipette from the beaker to the test tube.
3. Take the white liquid flower extract liquid filtrate using a pipette.
4. Squirt the solution of the test of into the liquid of each urine sample.
5. Observe the changes that occur in the urine after being dropped by the test solution
When living things are growing up, every individual will find a life partner to preserve his offspring. Just like humans, they will get married at the time they specify and want to have a child. For a woman, getting pregnant is the most awaited moment after marriage. Therefore, they are always ready to know early pregnancy. Knowing early pregnancy can feel the early signs of pregnancy. According to Muhtasor (2013), during the pregnancy period there will be changes in physical condition and physiological signs ranging from nausea and vomiting, headache to general complaints such as heartburn in the stomach especially. All of these symptoms can be ascertained that she is pregnant or there is a dangerous disease in her body. By knowing the pregnancy, immediately go to the doctor or midwife to observe the fetus in the stomach.
In the first experiment, the urine sample was dropped on the drop plate. Then the test solution of Test kits is also dropped. Physical changes were observed as shown in figure 4 above. The change in the UH + A sample is the test-kits solution directly spreading across the surface. Changes in UH + B samples are similar to those that occur in UH + A samples. The physical changes that occur in UH-Y samples are different from other sample changes. When the test-kits solution is dropped on a sample labeled UH-Y, there is clumping at the bottom of the drip plate.
The second experiment is done by placing the three samples into the test tube. This experiment was conducted to determine the visible physical changes from top to bottom of the test tube. The three urine samples were taken with a pipette and dripped into a test tube with the amount of liquid lumped. Each sample was dropped by one drop of test-kits solution. Then observed changes in physical reactions that occur therein. The change that occurs is the test-kits solution on the UH + A sample is not mixed and spread on the surface looks like water and oil separated. In the UH + B sample the same as the previous sample, the test solution of test-kits was not mixed and spread all over the surface. In the UH-Y sample the changes were very different from the previous two samples, ie thetest-kits test solution dropped directly down and and had clumped at the bottom of the test tube.
The last experiment uses the same place as the second experiment. The only difference is that it gives three drops of urinary test kitssolution. The observed result is the same as with the previous experiment. So, this experiment is only for retesting as well as proof for comparison on the first try. Of the three trials that have been done above, it is evident that red roses have a potential anti-HCG hormone content. The anti-HCG hormone seeks to remove the hormone HCG contained by the female urine pregnant and incompatible, so the test-kits solution stays on top and spreads quickly. Whereas, in normal female urine that does not contain HCG, the test solution of test-kits will instantly clump and fall to the bottom of the sample container.
The concept of work on the test-kits belongs to the type of indirect agglutination, this concept works by the presence of HCG in urine with an anti-HCG antibody (monoclonal) bonded to the reaction particles indicated by agglutination of the urine. This concept has an interpretation of the result that if positive pregnant, then agglutination does not occur. Whereas if the negative pregnant, then there will be agglutination.
The work concept of the test-kits almost resembles the Latex Agglutination Inhibition (LAI) and Hemaglutination Inhibition (HAI). When compared to the timing speed of detecting the clumping, test-kits takes only five seconds and then the results are clearly visible compared to LAI takes two minutes and HAI takes 1 – 2 hours.
Lailatul Qiroati and Lucky A.P.
Bungah Junior High School Gresik