Identification and Determination of Alkoloid Chemical Structure from Vernonia Amygdalina Delile Leaves and Their Effects on Glucosidase Enzyme in Diabetics

Identification and Determination of Alkoloid Chemical Structure from Vernonia Amygdalina Delile Leaves and Their Effects on Glucosidase Enzyme in Diabetics

The number of people with diabetes mellitus increases every year. WHO data shows that Indonesia fourth highest ranks in the world. Besides diabetes is a life-threatening disease of the sufferer. Traditional diabetes treatment has been carried out by the world community. One plant that has antidiabetic potential is the leaf of South Africa (Vernonia amygdalina delile). Large amounts of alkoloid are contained in South African leaves. Isolation and identification of leaves need to be done considering the phytopharmic activity of the broad alkoloid and influential in the metabolism of diabetics. While in vitro testing was conducted to determine the antidiabetic mechanism of action of alkoloid compounds isolated from South African leaf extracts (Vernonia amygdalina delile).
Identification and Determination of Alkoloid Chemical Structure from Vernonia Amygdalina Delile Leaves and Their Effects on Glucosidase Enzyme in Diabetics
South Africa Plant (Vernonia amygdalina delile)
This study aims to identify and determine the alkoloid structure of South African leaves and their effects on the inhibition of the enzyme a-glucosidase. This research method uses qualitative and quantitative methods. South African leaves are extracted by maceration using n-hexane and ethanol solvents. Alkoloid is isolated from ethanol extract acid-base. Alkoloid test was carried out by adding Meyer and drgendroff. White precipitate formed shows positive test results. Separation and settlement of compounds is done by the TLC method. Identification and determination of alkoloid structures using FTIR and LC-MS spectrophotometers. From the results of the identification analysis of compounds suspected compounds in alkoloid isolates are benzamide compounds (C17H14N2O2) with inhibitory activity against the enzyme a-glucosidase
Identification and Determination of Alkoloid Chemical Structure from Vernonia Amygdalina Delile Leaves and Their Effects on Glucosidase Enzyme in Diabetics
South Africa Leaf (Vernonia amygdalina delile)
Keywords: Alkoloid, enzyme a-glucosidase, South African leaf (Vernonia amygdalina delile), structural identification, Diabetes Mellitus

By: Ananda Aulia Parahita and Anasya Rahmawati
Al-Irsyad Al-Islamiyah IT High School Purwokerto Central Java
LPIR LIPI Jakarta

 

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